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葡萄糖对单针藻异养、兼养生长及油脂合成的影响

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利用Andrew方程考查了异养和兼养培养模式下葡萄糖对单针藻Monoraphidium sp.FXY-10生长的影响,拟合出葡萄糖消耗动力学模型;同时研究了不同培养模式下葡萄糖对单针藻油脂合成的影响,拟合出最佳葡萄糖浓度时油脂合成动力学模型.结果表明,葡萄糖浓度为10.0g/L时,异养、兼养培养条件下藻细胞生物量和油脂含量达到最大值;异养、兼养培养条件下葡萄糖的半饱和常数KS分别为17.82g/L和1.89g/L,说明兼养培养时单针藻对葡萄糖的吸收能力相对较高.
Effects of Glucose on Cell Growth and Lipid Synthesis of Monoraphidium sp.FXY-10 Under Mixotrophic and Heterotrophic Cultivations
The effects of initial glucose concentrations on cell growth of Monoraphidium sp.FXY-10 under mixotrophic and heterotrophic cultivations were investigated by Andrew equation.A kinetic model was developed to describe the dynamics of glucose consumption for FXY-10 growth.Meanwhile, the influence of different glucose concentrations on the production of lipid by FXY-10 was studied.And the kinetic models of FXY-10 lipid synthesis in the different trophic types with the most appropriate glucose concentration were also constructed.The results showed thatwhen the concentration of glucose was 10g/L, the maximum total lipid content and biomass concentration were achieved under mixotrophic and heterotrophic conditions.The semi-saturation constants of Monoraphidium sp.FXY-10 to glucose in the mixotrophic (KSJ) and heterotrophic (KSY)conditions were 1.89g/L and 17.82g/L, respectively.It implied that the glucose absorption capacity of FXY-10 in the mixotrophic condition was relatively higher than that in the heterotrophic.

Monoraphidium sp.FXY-10MixotrophicHeterotrophicGlucoseLipid content

车绕琼、黄力、王琳、赵鹏、李涛、余旭亚

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昆明理工大学生命科学与技术学院 昆明 650500

单针藻 兼养 异养 葡萄糖 油脂含量

国家自然科学基金资助项目

21266013

2015

中国生物工程杂志
中国科学院文献情报中心 中国生物技术发展中心 中国生物工程学会

中国生物工程杂志

CSTPCDCSCD北大核心
影响因子:0.589
ISSN:1671-8135
年,卷(期):2015.35(11)
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