Optimization for High Production Fermentation of Lunasin from Recombinant Pichia pastoris
Lunasin is a 43 amino acid polypeptide originally isolated from soybean with bioactive properties such as antihypertensive,antioxidant activity,cancer prevention and therapy,anti-inflammation,hypocholesterolemic activity,anti obesity and immunomodulation.In order to achieve efficient production of Lunasin,the methanol fed methods (DO feedback fedding and index-constant speed feeding) and induction temperature of recombinant Pichia pastoris GS115 LN were investigated.Furthermore,the feeding strategies of mixed carbon sources during induction phase were investigated.The results show that the best feeding strategies and induced temperature were index-constant speed feeding,24 ℃.Consequently,the highest expression of Lunasin was 3.27g / L by feeding 1％ soy peptone and 0.02％ aspartic acid,which was 1.27 times higher than the single methanol induction process.
LunasinRecombinant Pichia pastorisHigh density fermentationProcess optimization
NETL
NSTL
万方数据
维普
