Transcriptome Analysis and Differential Gene Mining of Perennial-root Sugarcane
Sugarcane ratooning ability is directly related to its production cost and cultivation efficiency.Sugarcane ratooning productivity is influenced by variety,environment and cultivation measures,but variety is the most critical factor.There are few reports explaining the differences in sugarcane ratooning ability at the molecular biological level both domestically and internationally.In this paper,transcriptome sequencing was used to analyze the differences in gene expression between sugarcane GR2 and ROC22 of different root years.The results showed that 100 558 transcripts and 25 582 unigenes were obtained by transcriptome sequencing.By functional annotation of unigenes,including comparison with NR,Swiss-Prot,KEGG,COG,KOG,GO and Pfam databases,a total of 53 790 unigene annotation results were obtained.The GO functional annotation was divided into three categories and 51 subcategories.A total of 1 029 differential genes were annotated in six-year-old sugarcane,and 3 391 differential genes were annotated in three-year-old sugarcane.There are eight major KEGG metabolic pathways,which are mainly involved in plant hormone signal transduction,starch and sucrose metabolism,glycine,serine and threonine metabolism,phenylpropanoid biosynthesis,homologous recombination,DNA replication,mismatch repair and plant pathogen interaction.Six abscisic acid-related differentially identified genes are:abscisic acid-insensitive 5-like protein 2(ABI2),putative bZIP transcription factor superfamily protein,protein abscisic acid-insensitive 5(ABI5),aba responsive element-binding factor 1(partial ABF1),G-box binding factor 4(GBFs),and aba responsive element-binding factor 1(ABF).The above differential genes will serve as reference genes for our later gene expression and function analysis,and further analyze the molecular mechanism affecting sugarcane ratooning ability by combining proteomics.
NETL
NSTL
万方数据
