Improving the Ability of Bacillus licheniformis to Produce Alkaline Protease by Inactivating Sec Pathway Repressor Protein and Extracellular Proteases
In order to investigate the influence of the Sec secretion pathway on alkaline protease production in Bacillus licheniformis,the molecular chaperone-blocking protein gene hrcA and the three extracellular protease genes epr,bpr,and vpr in the genome of B.licheniformis TCCC11470(BL△upp△eps△pgs)were sequentially deleted.By comparing the alkaline protease activities before and after gene deletion,it was found that the knockout strains TCCC11470△hrcA and TCCC11470△hrcA△epr△bpr△vpr reached alkaline protease activities of 18 521.2 U/mL and 20 048.5 U/mL after 42 hours,respectively,which were 27.9％and 38.5％higher than the control strain BL△upp△eps△pgs(14 478.6 U/mL),respectively.These results indicate that optimizing the Sec secretion pathway can effectively enhance the enzymatic activity of alkaline protease,providing new insights and research strategies for the construction of industrial enzyme production hosts.
NETL
NSTL
万方数据
