Establishment of an Efficient in Vitro Regeneration System for Mature Zygotic Embryos in Larix gmelinii
The mature zygotic embryos of Larix gmelinii were used as explants to study the isolated organogenesis of larch.The experimental design used different medium,including 6-BA and 2,4-D to induce healing and adventitious shoots,elongation and rooting.The results showed that the best callus induction medium was 1/2 MS+2 mg/L 2,4-D,and callus induction rate was 91.11％.Induction medium for adventitious budding was 1/2 MS+1 mg/L 6-BA+0.07 mg/L 2,4-D,and the adventitious shoot induction rate was 87.78％.The indefinite bud elongation medium was MS+0.20 mg/L 6-BA+0.01 mg/L 2,4-D+1 mg/L activated carbon,and the elongation rate of adventitious buds reached 48.33％.Adventitious root rooting medium was 1/2 MS+1 mg/L IB A,and the induction rate of indefinite root reached 36.67％.The survival rate of transplanted seedlings was 100％.The above studies provide a good theoretical and practical basis for larch tissue culture organogenesis and further research on stable genetic transformation.
Larix gmeliniiMature zygotic embryoRegeneration system
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