The mature zygotic embryos of Larix gmelinii were used as explants to study the isolated organogenesis of larch.The experimental design used different medium,including 6-BA and 2,4-D to induce healing and adventitious shoots,elongation and rooting.The results showed that the best callus induction medium was 1/2 MS+2 mg/L 2,4-D,and callus induction rate was 91.11％.Induction medium for adventitious budding was 1/2 MS+1 mg/L 6-BA+0.07 mg/L 2,4-D,and the adventitious shoot induction rate was 87.78％.The indefinite bud elongation medium was MS+0.20 mg/L 6-BA+0.01 mg/L 2,4-D+1 mg/L activated carbon,and the elongation rate of adventitious buds reached 48.33％.Adventitious root rooting medium was 1/2 MS+1 mg/L IB A,and the induction rate of indefinite root reached 36.67％.The survival rate of transplanted seedlings was 100％.The above studies provide a good theoretical and practical basis for larch tissue culture organogenesis and further research on stable genetic transformation.
Larix gmeliniiMature zygotic embryoRegeneration system
NETL
NSTL
万方数据
