Application of Small RNA Regulatory Tools in Cyanobacteria Synthetic Biology
The application of gene regulatory tools in the field of cyanobacterial synthetic biology is particularly important for controlling gene expression,engineering strain development,and the production of additional economic products.Small RNA regulatory tools,which are based on the principle of targeted specificity regulation of small RNAs,are quantitative and global regulatory tools that are combined with synthetic biology for specific regulation of target genes.These tools use small RNAs and their targets as interaction modules and use inducible expression switches,scaffold sequences,and partner proteins as ancillary modules.Such tools have been applied to the synthesis of high-value economic products and the modification of algal strains for fuel and chemical tolerance.This paper provides an overview of different types of small RNA regulatory tools,their regulatory principles,the selection and modification of ancillary modules,as well as research progress in the synthesis of additional economic products and improvement of cyanobacterial tolerance.It also discusses potential problems and future development prospects of small RNA tools in applications,providing a reference for the design of efficient and precise molecular editing tools.
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