Characterization of Anillin-related Protein and Its Function in Yarrowia lipolytica
Objective:To identify the anillin-related protein in Yarrowia lipolytica and to study its function in septin organization.Methods:We identified the anillin-related protein in Y.lipolytica by aligning the protein sequence of Saccharomyces cerevisiae Bud4 in the Y.lipolytica genome database.The sequences of these anillin-related proteins were amplified and fused with green fluorescent protein GFP to observe their intracellular localization.In the model organism budding yeast,these anillin-related proteins of Y.lipolytica were overexpressed and their effects on cell morphology and septin organization were determined to investigate their function in this process.Results:Two potential anillin-related proteins,named YlBud4A(protein number YALIOD11880 in the database)and YlBud4B(protein number YALIOB07623),were identified by sequence comparison in the Y.lipolytica genome database,both of which were conserved in their C-terminal containing DUF1709 and PH domain.YlBud4A had no bud neck localization,whereas YlBud4B localized to the bud neck as a double ring in medium and large bud cells,a localization pattern identical to Bud4.Overexpression of YlBud4B in budding yeast caused elongated buds and septin disruption,which clustered into dots.Conclusion:YlBud4B may interact with the septins and it is likely to be the functional homolog of anillin-related protein whereas YlBud4A is not.
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