Novel Gene Editing Technology Accelerates Maturation and Application of Dunaliella salina Expression Systems
Dunaliella salina(D.salina)has become a promising expression system in genetic engineering due to its low cost,easy cultivation,simple transgenic operation,and controllable transcription and translation characteristics.However,there are technical bottlenecks such as low expression and unstable inheritance in the D.salina expression system,which severely limit its application.With the advantages of high stability,the high target specificity and programmability,the CRISPR/Cas system can be used as an important tool to promote the improvement and application of the D.salina expression system,thereby greatly increasing the production of high value-added products such as recombinant proteins,lipid accumulation and carotene.Due to the dual characteristics of prokaryotic and eukaryotic cells,salt algae have greater difficulties and limitations in the gene editing process.Therefore,it is necessary to continuously improve the accuracy and editing efficiency of gene editing technology in D.salina to ensure that the target genes are accurately modified.On this basis,the advantages of genetic engineering of D.salina expression system were comprehensively reviewed,including the transformation methods,exogenous gene expression and the current research status of gene editing.Meanwhile,the main challenges of CRISPR/Cas editing technology in D.salina are highlighted,the future development direction and improvement strategy are proposed,and the application prospect of this technology is envisioned to accelerate the early maturation and popularization of D.salina expression system.
Dunaliella salinaNovel expression systemGene editingCRISPR/Cas9 system
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NSTL
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