生物纳米孔在氨基酸检测及序列识别的研究进展
Advances of Biological Nanopores in Amino Acid Detection and Sequence Recognition
郑玲 1王徐 1周硕 2陈萧寒 2王德强2
作者信息
- 1. 重庆理工大学药学与生物工程学院 重庆 400054;中国科学院重庆绿色智能技术研究院 重庆 400714;中国科学院大学重庆学院 重庆 400714
- 2. 中国科学院重庆绿色智能技术研究院 重庆 400714;中国科学院大学重庆学院 重庆 400714
- 折叠
摘要
氨基酸是生物体的重要组成物质,为生命活动提供了物质基础.由于氨基酸种类多样,可以通过脱水缩合反应生成多肽,并通过数个多肽盘曲折叠产生蛋白质,对多肽和蛋白质的结构和功能造成影响.因此,识别不同氨基酸并对氨基酸序列进行读取具有重要意义.纳米孔检测技术是一种新型单分子传感技术,具有无须标记、灵敏度高、检测速度快等优点,广泛应用于核酸、蛋白质和多糖等待测物的检测.综述了近年来生物纳米孔在氨基酸检测及氨基酸序列识别中的应用,具体囊括了单个氨基酸的识别区分、不同多肽和蛋白质的检测以及潜在氨基酸序列技术的开发,并对相关发展前景提出展望.
Abstract
Amino acids are essential components of living organisms,providing the basic building blocks for many life processes.They undergo dehydration-condensation reactions to form peptides,which are then coiled and folded into proteins.The structural and functional differences betweeen peptides and proteins are strongly influenced by different amino acid sequences.It is therefore of great significance to detect amino acids and identify the amino acid sequence.Nanopore technology is a novel single-molecule detection technology that offers the advantages of label-free detection,high sensitivity,and rapid analysis.It has been widely applied to the detection of nucleic acids,proteins,and polysaccharides.This article provides a comprehensive review of the application of nanopores in amino acid detection,peptide and protein detection,and amino acid sequence identification.Furthermore,the prospects in this field are also included.
关键词
生物纳米孔/单分子检测/氨基酸/多肽/蛋白质/测序Key words
Nanopore/Single molecule/Amino acids/Polypeptide/Protein/Sequencing引用本文复制引用
基金项目
重庆市自然科学基金(2022NSCQ-MSX1555)
出版年
2024
NETL
NSTL
万方数据