间充质干细胞外泌体抗肝纤维化的机制及其优化策略
Mechanism and Optimization Strategy of Mesenchymal Stem Cell Exosomes against Liver Fibrosis
周晓磊 1徐岩 1金煜 2叶俊松3
作者信息
- 1. 赣南医科大学 赣州 341000;赣南医科大学第一附属医院干细胞临床转化分中心 赣州 341000
- 2. 赣南医科大学 赣州 341000
- 3. 赣南医科大学第一附属医院干细胞临床转化分中心 赣州 341000;赣州市干细胞与再生医学重点实验室 赣州 341000;赣南医科大学江西省医用组织工程材料与生物制造重点实验室 赣州 341000;赣南医科大学心脑血管疾病防治教育部重点实验室 赣州 341000
- 折叠
摘要
肝纤维化是肝脏疾病治疗领域的一大难题.间充质干细胞可用于治疗肝纤维化,但因其可能的致癌性、促癌性及移植所需细胞量大等问题受到限制.近年来,间充质干细胞外泌体以更小的体积、更低的免疫原性、无致癌性等优势逐渐成为研究热点.然而,间充质干细胞外泌体在临床应用上也受到限制:首先,目前对不同种类间充质干细胞外泌体的提取与鉴定缺乏更为标准统一的方式;其次,间充质干细胞外泌体治疗肝纤维化的机制尚不清晰;最后,间充质干细胞外泌体治疗存在靶向活化的肝星状细胞(aHSC)能力弱、外泌体产量低、载药能力和递送效率低等问题.鉴于以上原因,对引起常见间充质干细胞外泌体提取效果差异的因素、间充质干细胞外泌体改善肝纤维化的机制及其优化策略进行综述,为间充质干细胞外泌体治疗肝纤维化提供新理解、新思路.
Abstract
Liver fibrosis is a major problem in the treatment of liver disease.Mesenchymal stem cells can be used to treat liver fibrosis,but are limited by their potential carcinogenicity,and the large number of cells required for transplantation.In recent years,mesenchymal stem cell exosomes have become a research hotspot due to their smaller size,lower immunogenicity and non-carcinogenicity.However,the clinical application of mesenchymal stem cell exosomes is limited for the following reasons.First,there is a lack of standard and uniform methods for extracting and identifying exosomes from different types of mesenchymal stem cells.Second,the mechanism of mesenchymal stem cell exosomes to treat liver fibrosis is not clear.Finally,mesenchymal stem cell exosome therapy has some problems such as weak ability to target aHSC,low exosome production,low drug loading ability and low delivery efficiency.In view of the above reasons,the factors causing the difference in exosome extraction effect of common mesenchymal stem cells,the mechanism of mesenchymal stem cell exosomes ameliorating liver fibrosis and its optimization strategy were reviewed,providing new understanding and new ideas for the treatment of liver fibrosis by mesenchymal stem cell exosomes.
关键词
间充质干细胞外泌体/肝纤维化/机制/优化策略Key words
Mesenchymal stem cell exosomes/Hepatic fibrosis/Mechanism/Optimization strategy引用本文复制引用
基金项目
国家自然科学基金(32060232)
江西省自然科学基金(20212BAB206075)
出版年
2024
NETL
NSTL
万方数据