丙谷二肽的合成研究进展
Advances in the Synthesis of Ala-Gln
全浩龙 1梁文豪 1柯崇榕 1杨欣伟 1黄建忠1
作者信息
- 1. 福建师范大学生命科学学院 工业微生物发酵技术国家地方联合工程研究中心工业微生物教育部工程中心 福州 350108
- 折叠
摘要
丙谷二肽(L-alanyl-L-glutamine,Ala-Gln)是一种重要的二肽,其在体内可分解为L-谷氨酰胺(L-Gln),该氨基酸具有不可替代的生理生化作用.传统化学法合成Ala-Gln存在污染高、转化率低、纯度低等缺点,并伴随有毒副产物产生,这限制了其规模化应用.随着生物学的快速发展,研究者们解析了 Ala-Gin的合成方式,并对菌株进行了定向改造,为绿色高效生产Ala-Gln开启了新纪元.主要对Ala-Gln的应用、传统化学法和生物法合成Ala-Gln的研究进展进行综述,旨在探索如何利用合成生物学、基因组学等学科技术改造Ala-Gln的代谢途径,高效生产Ala-Gin,为Ala-Gln产业化提供理论基础.
Abstract
L-Alanyl-L-Glutamine(Ala-Gln)is an important dipeptide that can be decomposed as L-glutamine(L-Gln)in the body,which plays an irreplaceable physiological and biochemical roles.The traditional chemical synthesis method suffers from the disadvantages of high pollution,low conversion,and low purity,and is accompanied by the generation of toxic by-products,which limits its large-scale application.With the rapid development of biology,researchers have analyzed the synthetic methods of Ala-Gln and directed the modification of strains,which opens up a new era for the green and efficient production of Ala-Gln.This paper reviews the application of Ala-Gln,the metabolic pathways of Ala-Gln synthesized by traditional chemical synthesis and microbial synthesis,and the progress of microbial production of Ala-Gln,with the aim of exploring the use of synthetic biology to optimize the metabolic pathway of Ala-Gln,providing a theoretical basis for the efficient production of Ala-Gln,and promoting the industrial production of Ala-Gln.
关键词
丙谷二肽/化学合成/生物合成/L-氨基酸连接酶/α-氨基酸酯基转移酶Key words
Ala-Gln/Chemical synthesis/Biosynthesis/L-amino acid ligase/α-Amino acid ester acyltransferase引用本文复制引用
基金项目
国家自然科学基金面上项目(22377012)
福建省自然科学基金面上项目(2021J01170)
福建省自然科学基金面上项目(2021J01172)
出版年
2024
NETL
NSTL
万方数据