目的:微生物是降解磺胺类抗生素(sulfonamides,SAs)的主要驱动者,但在抗生素胁迫下易处于活的非可培养状态(viable but non-culturable,VBNC)而无法筛选得到;利用藤黄微球菌产生的复苏促进因子(resuscitation-promoting factor,Rpf)改善VBNC降解菌群的生长繁殖特性,提高废水的抗生素去除效果.方法:以磺胺二甲嘧啶(sulfamethazine,SMZ)废水为处理对象,利用Rpf蛋白的复苏作用驯化SMZ废水处理系统内活性污泥中潜在的功能降解菌群.结果:当SMZ质量浓度为0.5mg/L时,投加Rpf蛋白后,SMZ的去除率提高了 9.38％;当SMZ质量浓度增加到20 mg/L时,SMZ的去除率提高了 17.10％;高通量测序结果表明,Rpf蛋白的投加主要促进了与SMZ降解相关的归属于变形菌门(Proteobacteria)和拟杆菌门(Bacteroidota)的unclassified_f_Comamonadaceae、OLB8、Shinella和Rhodococcus等在系统内的富集,进而提高了 SMZ去除效果.结论:首次利用Rpf强化微生物降解SMZ废水,为Rpf应用于其他磺胺类抗生素的去除提供了理论基础.
Synergistic Treatment of Sulfamethazine Wastewater by Resuscitation-promoting Factor Rpf
Objective:Microorganisms are the main drivers of the degradation of sulfonamide antibiotics(SAs),but they are hardly screened because they are easily in a viable but non-culturable(VBNC)state under the antibiotic stress.The resuscitation-promoting factor(Rpf)produced by Micrococcus luteus can effectively improve the growth and reproduction characteristics of VBNC-degrading bacteria and further increase the removal rate of antibiotic wastewater.Methods:This study focuses on the treatment of sulfamethazine(SMZ)wastewater,and utilizes the recovery effect of Rpf protein to domesticate the potential functional degrading bacteria in the activated sludge during the SMZ treatment system.Results:When the concentration of SMZ was 0.5 mg/L,the addition of Rpf protein increased the removal rate of SMZ by 9.38％.When the concentration of SMZ was further increased to 20 mg/L,the degradation rate of SMZ increased by 17.10％compared with the group without Rpf.The high-throughput sequencing results revealed that the addition of Rpf protein mainly promoted the enrichment of key microorganisms related to SMZ degradation,such as unclassified_f_Comamonadaceae,OLB8,Shinella,and Rhodococcus,which belong to Proteobacteria and Bacteroidota,thereby improving the SMZ removal efficiency.Conclusion:In this study,Rpf was used for the first time to enhance the microbial degradation of SMZ wastewater,providing a theoretical basis for the application of Rpf in the removal of other sulfonamide antibiotics.
万方数据
维普
