首页|工业用寡聚酶的改造与设计研究进展

工业用寡聚酶的改造与设计研究进展

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在自然界中,绝大多数酶具有寡聚特性.寡聚酶在工业上具有重要的价值,它能够提高反应速度和效率,降低生产成本,减少废物产生,扩大反应底物范围,提高产品质量.这些优势使得寡聚酶成为工业生产中不可或缺的催化剂.但是,寡聚酶在催化过程中,亚基解离可能导致酶活性减弱,这是其工业化应用的主要限制因素之一.因此,提升工业用寡聚酶的催化稳定性是当前生物催化与转化研究领域的重要课题.近年来,研究人员致力于通过优化手段提高寡聚酶的稳定性和催化活性以满足工业应用的需求.综述工业用寡聚酶的普遍性、寡聚化调节过程、功能影响以及分子改造情况,同时对未来工业化利用寡聚酶的发展前景进行展望,以期实现对寡聚酶催化和反应性能的精确调控,为高效工业用酶战略的发展提供理论指导.
Research Progress on Modification and Design of Some Valuable Oligomers in Industry
In nature,most enzymes have oligomerization properties and have been widely used in industrial production.In industry,oligomers have important value.They can improve reaction speed and efficiency,reduce production costs,reduce waste generation,expand the range of reaction substrates,and improve product quality.These advantages make oligomers an indispensable catalyst in industrial production.However,in the process of oligomer catalysis,subunit dissociation can lead to the weakening of the enzyme activity,which is one of the main limiting factors for its industrial application.Therefore,improving the catalytic stability of industrial oligomers is an important topic in the field of biocatalysis and transformation.In recent years,researchers have focused on improving the stability of these enzymes through optimization to meet the needs of industrial applications.In this review,the generality,regulation of oligomerization,functional effects and molecular modification of industrial oligomers were reviewed.At the same time,the development prospects of the industrial utilization of oligomers in the future are discussed to achieve accurate regulation of their catalytic and reaction properties,and to provide theoretical guidance for the development of efficient industrial application enzyme strategy.

Valuable oligomers in industryStabilityCatalytic activityMolecular modification

刘玉芬、刘森

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湖北工业大学工业发酵省部共建协同创新中心 武汉 430068

湖北微生元生物科技有限公司 鄂州 436006

湖北工业大学发酵工程教育部重点实验室 武汉 430068

工业用寡聚酶 稳定性 催化活性 分子改造

国家自然科学基金湖北省杰出青年基金湖北省创新群体项目工业发酵省部共建协同创新中心开放基金

319711502019CFA0692024AFA3042023SL01

2024

中国生物工程杂志
中国科学院文献情报中心 中国生物技术发展中心 中国生物工程学会

中国生物工程杂志

CSTPCD北大核心
影响因子:0.589
ISSN:1671-8135
年,卷(期):2024.44(7)
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