一株彭氏变形杆菌噬菌体phiP4-3生物学特性及基因组学研究
Biological Characteristics and Genomic Analysis of a Lytic Phage phiP4-3 Specific for Proteus penneri
何洋 1李东航 2荆兆元 2杨洪江2
作者信息
- 1. 杰科(天津)生物医药有限公司 天津 300467;工业发酵微生物教育部重点实验室天津市工业微生物重点实验室天津科技大学生物工程学院 天津 300457
- 2. 工业发酵微生物教育部重点实验室天津市工业微生物重点实验室天津科技大学生物工程学院 天津 300457
- 折叠
摘要
背景:彭氏变形杆菌是重要的致病菌,其耐药菌株的出现给公共卫生和食品安全带来了严重威胁.噬菌体能杀死病原菌,具有替代抗生素的潜在能力.目的:以彭氏变形杆菌(Proteus penneri)为宿主菌,从淡水鱼肠道中分离得到一株烈性噬菌体phiP4-3,并对其生物学特性、基因组学和分类学进行了研究.方法:使用透射电子显微镜观察噬菌体的形态;通过一步生长曲线和抑菌曲线,分析噬菌体的杀菌效果;通过基因组测序、注释和比较基因组学分析噬菌体的功能和分类.结果:通过透射电子显微镜观察,phiP4-3噬菌体头部直径为98.83 nm,可伸缩尾部长度为55.35 nm.一步生长曲线结果显示,该噬菌体的潜伏时间约为25 min,每个感染中心释放的噬菌体数量约为17 PFU.抑菌曲线结果显示,噬菌体phiP4-3能有效抑制宿主菌生长.基因组测序组装结果显示,噬菌体phiP4-3的基因组长度为167 849 bp,GC含量为31.5％.使用软件对基因组进行注释,发现噬菌体phiP4-3的基因组上共编码270个ORF和8个tRNA基因.综合比较基因组学、分类学及国际病毒分类委员会(ICTV)的分类结果,将其归类为Straboviridae科Bragavirus属病毒.结论:分离得到一株新彭氏变形杆菌噬菌体,并对其生物学特性和基因组进行了分析,为噬菌体在公共卫生和食品安全中的应用提供了理论依据.
Abstract
Background:Proteus penneri is an important bacterial pathogen,and the emergence of the multidrug resistant strains poses a major threat to public health and food safety.Phages can kill the host bacterial pathogens and are possible alternatives to conventional antibiotics.Objective:In this study,a lytic phage phiP4-3 was isolated from the gastrointestinal contents of freshwater fish using a Proteus penneri strain as an indicator.Phage phiP4-3 was investigated for biological characteristics,genomics,and taxonomy.Methods:The morphology of phiP4-3 was analyzed by transmission electron microscope(TEM);the bactericidal effect of phage was analyzed by one-step growth curve and bacteriostatic curve;the function and classification of phages were analyzed by genome sequencing and comparative genomics.Results:Through TEM observation,the head diameter of phiP4-3 bacteriophage is 98.83 nm,and the retractable tail length is 55.35 nm.The results of the one-step growth curve show that the incubation time of the phage is about 25 min,and its burst size was about 17 PFU/infection center.The antibacterial curve results showed that phiP4-3 can effectively inhibit the growth of the host.The genome sequencing assembly results showed that the genome length of phiP4-3 was 167 849 bp,with a GC content of 31.5％.Bioinformatic tools reveal that the phiP4-3 genome has 270 ORFs and 8 tRNA genes,and no antibiotic resistance genes or virulence genes were identified.Based on the classification results of comparative genomics,taxonomy,and ICTV,the phiP4-3 can be classified in the family Straboviridae and genus Bragavirus.Conclusion:A phage from Proteus penneri was isolated,and its biological characteristics and genomics were analyzed,providing a theoretical basis for the application of bacteriophage in public health and food safety.
关键词
彭氏变形杆菌/噬菌体phiP4-3/基因组学/比较基因组学/分类学Key words
Proteus penneri/Phage phiP4-3/Genomics/Comparative genomics/Taxonomy引用本文复制引用
出版年
2024
NETL
NSTL
万方数据