Biological Characteristics and Genomic Analysis of an Ackermannviridae LHE287 Isolated from Beagle Farm
Objective:To isolate a phage from environmental samples of the beagle kennel,and identify its biological characteristics and genomic characterization.Methods:96 clinical isolates of Escherichia coli(E.coli)were used as host bacteria.Phage LHE287 was isolated by the double-layer plate method.Its morphology was observed bytransmission electron microscope.The host range,multiplicity of infection(MOI),stability,one-step growth curve,and in vitro antibacterial activity of phage LHE287 were determined by the double-layer plate method.The genomic characteristics of phage LHE287 were analyzed.Results:A bacteriophage LHE287 with a lysis ability against E.coli and Salmonella was isolated from canine feces.It has an icosahedral head(～86 nm)and a retractable tail(～107 nm)with a forked umbrella-like tip,and was determined to be a member of the Ackermannviridae family.The genome of phage LHE287 was 149 485 bp,including 168 open reading frames(ORFs)and three tRNAs,and no virulence or resistance gene was found.E.coli 287 was used as a host strain for the proliferation of phage LHE287 with an incubation time of 30 min and a burst size of 100 PFU/cell.Phage LHE287 had the highest titer of 1.72 × 109 PFU/mL at a MOI of 0.000 1.It remained stable up to 50℃ and the range of pH 5-10,and showed a resistance to ultraviolet light.Conclusions:An Ackermannviridae phage,LHE287,was successfully isolated,showing considerable potential for further application.
NETL
NSTL
万方数据
