Characterization and Complete Genome Analysis of a Phage Infecting Methicillin-Resistant Staphylococcus aureus
Objective:Methicillin-resistant Staphylococcus aureus is one of the most important foodborne pathogens.The in-depth study of phage is of great value in the treatment of MRSA infection.Methods:MRSA38 was used as the host and a new phage was isolated from hospital sewage.The biological characteristics of the newly isolated phage were determined and its whole genome sequence was analyzed.The antibacterial effect of this phage on MRSA38 was assessed in vitro.Results:A new phage infecting MRSA38 was successfully isolated and named BUCT_X001.It has a 50 nm head and a 250 nm tail.It has an optimal multiplicity of infection of 0.01.The one-step growth curve shows that BUCT_X001 had a latency of 15 min,a rise phase of 150 min,and a burst size of 54.7 PFU/cell.BUCT_X001 can maintain more than 90％activity over a wide pH(5.0 to 9.0)and temperature(4℃ to 45℃)range.Whole genome analysis of BUCT_X001 reveals that it has a total length of 42 649 bp,with a(G+C)％content of 34％and it is predicted to encode 68 open reading frames.Phylogenetic tree results show that BUCT_X001 is a novel bacteriophage of the subfamily Bronfenbrennervirinae in the family Caudoviricetes.Conclusions:A new phage targeting MRSA was isolated and showed strong antibacterial effects on the growth of host bacteria.It has great potential for use in the prevention and treatment of infections caused by MRSA in clinical settings.
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