Effects of Cell Membrane Biomimetic Gold Nanoparticles on the Phenotype and Ultrastructure of Membrane-derived Cells
Objective:To study the effects of cell membrane biomimetic gold nanoparticles on cell phenotypes such as proliferation,growth and apoptosis as well as cell ultrastructure during the interactions between cell membrane biomimetic gold nanoparticles and membrane-derived cells.We will then evaluate the coexistence status of nanoparticles and cells.Methods:Hepatic stellate cell membrane biomimetic gold nanoparticle(AuNP@HSCM)were prepared using a mixed mechanical extrusion method.High-resolution transmission electron microscope was used to characterize the nano-biomimetic membrane interaction structure of AuNP@HSCM,and to analyze the subcellular ultrastructural changes of LX-2 hepatic stellate cells before and after treatment with AuNP@HSCM.Optical microscopy,flow cytometry and the CCK-8 method were used to determine the growth status,proliferation and apoptosis phenotypic characteristics of LX-2 hepatic stellate cells.Results:The cell membrane and nanoparticle surface of AuNP@HSCM are well coupled.Compared with polyethylene glycol-modified gold nanoparticles(AuNP@PEG),AuNP@HSCM can be transported to the cell nucleus and has a stronger cellular internalization ability.Unlike AuNP@PEG,which caused the degradation of organelle structures in the cytoplasm,AuNP@HSCM induced bleb-like structures near the cell membrane in LX-2 cells.Compared with AuNP@PEG acting on LX-2 cells,AuNP@HSCM has weaker cytotoxicity at low dose concentrations(≤1.5 μg/mL)and can significantly reduce the apoptosis level of LX-2 cells.Conclusion:Cell membrane biomimetic gold nanoparticles AuNP@HSCM have a stable nano-biomimetic membrane coupling structure,and can better achieve structural and functional coexistence with the membrane-derived cell LX-2.
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维普
