Preparation,Characterization and Antioxidant Activity of Pullulan-polyphenol Conjugate
Objective:Pullulan is a microbial polysaccharide with excellent biocompatibility.It has found applications in cosmetics as a moisturizer and thickener.Nevertheless,its limited bioactivity hampers its broader application.Methods:In this study,a pullulan-polyphenol conjugate was synthesized by a mild chemical reaction aimed at enhancing its biological activity.Pullulan was activated by oxidation with sodium periodate followed by reduced amination involving an active amino group.The activated pullulan was grafted with polyphenol through a dehydration condensation reaction catalyzed by EDC,resulting in the formation of the polyphenol conjugate.Results:The successful grafting of polyphenols onto pullulan was confirmed by UV spectroscopy,Fourier transform infrared spectroscopy,and 1H NMR.In vitro comparisons with native pullulan or polyphenols revealed that the pullulan-polyphenol conjugate exhibits enhanced thermal stability,antioxidant activity and moisturizing properties.Additionally,it demonstrated a commendable protective and reparative effect on human immortalized keratinocytes against oxidative damage.Conclusion:The pullulan-polyphenol conjugate has comparable antioxidant and moisturizing properties.It can be developed as a cosmetic raw material.
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