Enzyme Immobilization on Wood-derived Cellulose Scaffolds for Degradation of Dye Molecules
Objective:The use of immobilized enzyme technology overcomes the disadvantages of soluble enzymes in industrial applications,such as difficult recovery,environmental sensitivity and high cost,improving enzyme environmental stability and reusability of enzymes and reducing application costs.Methods:Balsa wood was selected as the raw material for the carriers,and the wood-based carriers were prepared by removing the lignin.The wood-based carriers were modified and immobilized with horseradish peroxidase(HRP)using citric acid modification,glutaraldehyde activation,and a combination of the two treatments,respectively.The functional groups and structures of the original balsa wood,wood,and HRP@wood were investigated using Fourier infrared spectroscopy(FT-IR)and scanning electron microscopy(SEM).Conclusion:The wood-based carrier with the combination of the two treatments had a higher immobilization of the enzyme up to 43.0 mg/g,and this immobilized enzyme material was named HRP@wood.Three dyes,crystal violet,methylene blue and Congo red,were selected to test the static decolorization performance of HRP@wood,and the optimal decolorization conditions of HRP@wood for a variety of dyes were determined by investigating the effects of dye concentration,H2O2 concentration and reaction time on the decolorization effect of the dyes.Results:The dyes were decolored at up to 76.9％,78.2％,and 82.4％,respectively in the first half hour when decolorized under ideal circumstances,which included 12 mg/L crystal violet and 2 mmol/L H2O2,4 mg/L methylene blue and 0.5 mmol/L H2O2,and 100 mg/L Congo red and 2 mmol/L H2O2.The decolorization rate of methylene blue can reach 100％when the decolorization period was increased to 120 mins.A dynamic decolorization reactor was constructed with this HRP@wood,and the results showed that the reactor had excellent recyclability and decolorization effect.
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