Mechanisms of Piceatannol in Alleviating Inflammatory Injury in Zebrafish
Prolonged or excessive inflammatory responses can cause serious damage to the human body,and preliminary experiments have shown that the small molecule drug piceatannol(PCT)can be used as a potential anti-inflammatory drug.In this study,reactive oxygen species detection assay was used,and it was found that the alleviation of inflammatory injury in zebrafish gradually increased with the increase of PCT concentration within a certain safety range,and there was a dose-dependent effect on the degree of alleviation,indicating that PCT has an antioxidant effect.The effects on CuSO4-induced cellular damage or oxidative stress-induced inflammation were investigated by using neutrophil-and macrophage-labelled transgenic zebrafish line Tg(coro1a:GFP;lyz:Dsred),in which zebrafish with CuSO4-induced neuroinflammation were treated with different concentrations of PCT,and the number of neutrophils migrating to the site of the spinal neuronal hillock was significantly reduced,indicating that PCT has an antioxidant effect on the neuroinflammation.The number of neutrophils migrating to the site of vertebral neuromast was significantly reduced,indicating that PCT has a restorative effect on the aggregation of inflammatory chemotactic neutrophils at the site of injury.Meanwhile,the qPCR results demonstrated that PCT treatment significantly down-regulated the expression of inflammatory marker genes such as IL-6,COX-2 and TNF-α compared with the CuSO4 treatment group alone,thus protecting zebrafish from CuSO4-induced inflammatory injury.Our study demonstrated the anti-inflammatory and antioxidant effects of PCT in the zebrafish model organism,providing a theoretical basis for the future use of PCT as an anti-inflammatory drug.
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