Functional Study of Hypoxia-inhibitory Factor ZNF92 in the Growth and Invasion of Hepatocellular Carcinoma
Objective:To investigate the function and mechanism of the hypoxia-regulated factor zinc finger proteins 92(ZNF92)in hepatocellular carcinoma cell growth and invasion.Methods:(1)The mRNA and protein expression levels of ZNF92 were detected by RT-qPCR and Western blot.(2)ZNF92 knockdown hepatocellular carcinoma cell line was established using lentiviral vectors.(3)The effects of ZNF92 on hepatocellular carcinoma HepG2 cells,and SMMC7721 cell growth and invasion in vitro were evaluated by CCK-8,scratch and transwell assays.(4)Small interfering RNA(siRNA)was used to determine the knockdown of ZEB1 expression and to determine whether ZNF92 indirectly regulates the epithelial-mesenchymal transition(EMT)process through ZEB1.(5)siRNA was used to determine the knockdown of HIF-1α and to detect the changes in cell migration and invasive ability.Results:(1)Hypoxia inhibited the mRNA and protein expression of ZNF92.(2)ZNF92 regulates the protein expression of zinc finger E-box binding homeobox 1(ZEB1),a key transcription factor of EMT,thereby affecting the EMT process.(3)ZNF92 inhibits hepatocellular carcinoma migration and invasion without affecting the proliferation process.(4)ZNF92 partially regulates migration and invasion of hepatocellular carcinoma cells through ZEB1.(5)ZNF92 may partially regulate ZEB1 through HIF-1α to affect the migration and invasion of hepatocellular carcinoma cells.Conclusions:The novel hypoxia-inhibitory factor ZNF92 may affect the EMT process through HIF-1 α,and then affect the migration and invasion of hepatocellular carcinoma cells by regulating ZEB1.
万方数据
维普
