Abiotic Stress Response of Two Transcripts of WRKY28-2 from Caragana korshinskii
Alternative splicing refers to the process in which different mRNA splice isomers are produced from an mRNA precursor by different splicing methods.WRKY transcription factors are one of the largest transcription factor families and are widely involved in growth,development,aging and abiotic stress processes.In this study,we found that WRKY28-2 from Caragana korshinskii can undergo alternative splicing and produce two different transcripts,CkWRKY28-2 and CkWRKY28-2-AS.The start codon of CkWRKY28-2 is ATG,the end codon is TGA,encoding 265 aa,the molecular weight of CkWRKY28-2 protein is 30 028.18 Da,and the pI is 8.16.The start codon of CkWRKY28-2-AS is ATG,the stop codon is TGA,encoding 68 aa,the molecular weight of CkWRKY28-2-AS is 7 286.71 Da,and the pI is 3.98.The results of RT-qPCR analysis showed that CkWRKY28-2 was mainly expressed in roots and stems,while CkWRKY28-2-AS was mainly expressed in leaves.Under drought stress,CkWRKY28-2 expression increased while CkWRKY28-2-AS expression decreased.Under salt stress,the expression levels of CkWRKY28-2 and CkWRKY28-2-AS increased with increasing salt treatment time.Under high temperature stress,the expression levels of CkWRKY28-2 and CkWRKY28-2-AS were significantly down-regulated compared with the control.Under low temperature stress,the expression levels of CkWRKY28-2 and CkWRKY28-2-AS decreased significantly with increasing treatment time.This study laid a foundation for the molecular mechanism of CkWRKY28-2 involved in stress resistance.
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