Screening of Mercury-tolerant Strains and Transcriptome Analysis under Mercury Stress
Various microorganisms in nature provide a vast library of genetic elements for heavy metal bioadsorption.The analysis of the mechanism of microbial heavy metal resistance and the study of related genes are prerequisites for the use of these genetic elements.In this study,we screened five highly mercury-tolerant strains from mercury-contaminated soils in mining areas.The genetic relationship of each strain was investigated by 16S rRNA sequencing analysis.The mercury tolerance of the strains was further tested.HG5,the closest relative of Peribacillus frigoritolerans,was the more mercury tolerant strain,still able to grow colonies in the medium containing 150 mg/L HgCl2.Transcriptome analysis of HG5 under mercury stress showed that nucleotide synthesis and metabolism,heme synthesis and metabolism,pyruvate metabolism,TCA cycle and other pathways were affected by mercury.At the same time,quantitative real-time PCR proved that mercury stress had a major impact on the sulfur assimilation process of HG5.After mercury stress,the expression of the trehalose operon gene increased significantly.This study could provide a theoretical basis for the utilization of heavy metal resistance genes and related gene elements.
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