Mammalian Exosomes:Isolation and Purification,Clinical Applications and Prospects
Exosomes,nanoscale extracellular vesicles secreted by cells,play a critical role in mediating intercellular communication and transporting a variety of bioactive substances,including nucleic acids,proteins,and lipids.These vesicles play a significant role in both physiological and pathological processes of the body.Due to their endogenous origin and biocompatibility,exosomes offer distinct advantages over synthetic carriers such as liposomes and nanoparticles,particularly in the area of disease diagnostics,biomarker discovery,and therapeutic applications.However,their clinical utility is currently hampered by challenges such as low efficiency in isolation and purification,and limited targeting capabilities.This article provides a comprehensive overview of exosomes,including their origin,classification,and recent advances in isolation and purification techniques.It critically evaluates the strengths and limitations of these methods.Additionally,the review discusses the burgeoning applications of exosomes in disease diagnostics and therapeutics,highlighting their potential and future prospects.By providing novel insights and strategies,this review aims to pave the way for more effective use of exosomes in clinical and research settings,thereby unlocking their full potential for healthcare innovation.
ExosomesExosome isolationClinical diagnosisTargeted drug deliveryTherapy
NETL
NSTL
万方数据
