Expression and purification of IFNβ-HAS fusion protein in Pichia pastoris
In order to obtain enough fusion protein for developing preclinieal studies of IFNβ-HAS, we screened Pichia pastoris transformants expressing high-level protein by immunology method.The yield of IFNβ-HSA was about 500 mg/L by fed-batch fermentation.The purity of IFNβ-HSA reached 96% through the steps of ultrafiltration, Blue Sepharose FF, Ni~(2+)-IMAC and DEAE Sepharose FE Analysis of Western blotting showed that IFNβ-HSA had the antigenicity of IFNβ and HSA.The specific activity was about 1.96×10~7 IU/mg by standard survival activity test on WISH cells challenged with VSV virus.This study provided a method to produce IFNβ-HSA.
NETL
NSTL
万方数据
维普
