Enhancing ethanol production using thermophilic yeast by response surface methodology
We optimized the conditions of simultaneous saccharification and fermentation(SSF)from cassava flour into high-concentration ethanol by thermophilic yeast GXASY-10.Based on the single factor experiment,we screened the important parameters by Plackeet-burman design.We used the path of steepest ascent to approach to the biggest region of ethanol production subsequently.Then,We obtained the optimum values of the parameters by Box-Behnken design.The results showed that the important parameters were the liquefaction time,glucosidase dosages and initial concentration of cassava flour(substrate).The optimum technical conditions were as follows:liquefaction time 35 rain.glucosidase dosages 1.21 AGU/g substrate and initial substrate concentration 37.62%.Under such optimum conditions,the ethanol yield of 20 L fermentor reached 16.07%(V/W)after 48 h fermentation at 37℃ and 100 r/min.The ethanol content increased 33% than that under the original condition.
fuel ethanolcassavasimultaneous saccharification and fermentation(SSF)Plackett-Burman designBox-Behnken design
NETL
NSTL
万方数据
维普
