Production of β-carotene by metabolically engineered Saccharomyces cerevisiae
β-carotene has a wide range of application in food,pharmaceutical and cosmetic industries.For microbial production of β-carotene in Saccharomyces cerevisiae,the supply of geranylgeranyl diphosphate (GGPP) was firstly increased in S.cerevisiae BY4742 to obtain strain BY4742-T2 through over-expressing truncated 3-hydroxy-3-methylglutaryl-CoA reductase (tHMGR),which is the major rate-limiting enzyme in the mevalonate (MVA) pathway,and GGPP synthase (GGPS),which is a key enzyme in the diterpenoid synthetic pathway.The β-carotene synthetic genes of Pantoea agglomerans and Xanthophyllomyces dendrorhous were further integrated into strain BY4742-T2 for comparing β-carotene production.Over-expression of tHMGR and GGPS genes led to 26.0-fold increase of β-carotene production.In addition,genes from X.dendrorhous was more efficient than those from P.agglomerans for β-carotene production in S.cerevisiae.Strain BW02 was obtained which produced 1.56 mg/g (dry cell weight) β-carotene,which could be used further for constructing cell factories for β-carotene production.
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