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越南金花茶叶绿体基因组分析

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本研究通过高通量测序技术对越南金花茶(Camellia insularis Orel&Curry)的叶绿体(chloroplast,cp)基因组进行了测序.结果显示,越南金花茶叶绿体基因组长度为 156 882 bp,包含132个基因,其中,88个编码蛋白质,36个编码tRNA,8个编码rRNA.密码子偏好性分析显示,编码亮氨酸的密码子数量最多,且第三位密码子显示出了较高的A/U偏好.此外,共鉴定出了67个简单重复序列(simple sequence repeats,SSR),发现越南金花茶SSR偏好使用A和T碱基.除了少数可变区域之外,越南金花茶与其近缘物种的叶绿体基因组反向重复(inverted repeat,IR)边界区域相当保守.系统发育分析表明,越南金花茶与云南金花茶(Camellia fascicularis)亲缘关系最近.金花茶是基因工程培育黄色山茶花的重要材料,本研究为叶绿体工程提供了基本的遗传信息,为深入研究金花茶组植物的进化、物种鉴定和基因组育种研究提供了宝贵的资源.
Characteristics of the chloroplast genome of Camellia insularis
In this study,the chloroplast genome of Camellia insularis Orel&Curry was sequenced using high-throughput sequencing technology.The results showed that the chloroplast genome of C.insularis was 156 882 bp in length with a typical tetrad structure,encoding 132 genes,including 88 protein-coding genes,36 tRNA genes,and 8 rRNA genes.Codon preference analysis revealed that the highest number of codons coded for leucine,with a high A/U preference in the third codon position.Additionally,67 simple sequence repeats(SSR)loci were identified,with a preference for A and T bases.The inverted repeat(IR)boundary regions of the chloroplast genome of C.insularis were relatively conserved,except for a few variable regions.Phylogenetic analysis indicated that C.insularis was most closely related to C.fascicularis.Yellow camellia is a valuable material for genetic engineering breeding.This study provides fundamental genetic information on chloroplast engineering and offers valuable resources for conducting in-depth research on the evolution,species identification,and genomic breeding of yellow Camellia.

Camellia insularis Orel&Currychloroplast genomestructural characteristicphylogenetic analysis

张进、邓永彪、赵博

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桂林医学院药学院生药学教研室,广西 桂林 541199

越南金花茶 叶绿体基因组 结构特征 系统发育分析

国家自然科学基金广西壮族自治区自然科学基金广西研究生教育创新计划

320600902021JJA130119YCSW2023414

2024

生物工程学报
中国科学院微生物研究所 中国微生物学会

生物工程学报

CSTPCD北大核心
影响因子:0.641
ISSN:1000-3061
年,卷(期):2024.40(1)