Characteristics of the chloroplast genome of Camellia insularis
In this study,the chloroplast genome of Camellia insularis Orel&Curry was sequenced using high-throughput sequencing technology.The results showed that the chloroplast genome of C.insularis was 156 882 bp in length with a typical tetrad structure,encoding 132 genes,including 88 protein-coding genes,36 tRNA genes,and 8 rRNA genes.Codon preference analysis revealed that the highest number of codons coded for leucine,with a high A/U preference in the third codon position.Additionally,67 simple sequence repeats(SSR)loci were identified,with a preference for A and T bases.The inverted repeat(IR)boundary regions of the chloroplast genome of C.insularis were relatively conserved,except for a few variable regions.Phylogenetic analysis indicated that C.insularis was most closely related to C.fascicularis.Yellow camellia is a valuable material for genetic engineering breeding.This study provides fundamental genetic information on chloroplast engineering and offers valuable resources for conducting in-depth research on the evolution,species identification,and genomic breeding of yellow Camellia.