The CRISPR sensing and detection technology has the advantages of cheap,simple,portable,high sensitivity,and high specificity,therefore is regarded as the"next-generation molecular diagnostic technology".Due to the specific recognition,cis-cleavage and nonspecific trans-cleavage capabilities,CRISPR-Cas systems have been implemented for the detection of nucleic acid targets(DNA and RNA)as well as non-nucleic acid targets(e.g.,proteins,exosomes,cells,and small molecules).This review summarizes the current CRISPR sensing and detection technologies in terms of the activity characteristics of different Cas proteins,with the aim to understand the advantages and development history of different CRISPR sensing and detection technologies,as well as promote its development and application.Moreover,this review summarizes the applications of various CRISPR sensing and detection technologies according to the types of detection targets,hoping to facilitate the development of novel CRISPR sensing detection technology.
关键词
规律间隔成簇短回文重复序列(CRISPR)/生物传感器/即时检测/分子诊断
Key words
clustered regulatory interspaced short palindromic repeats(CRISPR)/biosensors/instant detection/molecular diagnostics
维普
万方数据