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CRISPR传感检测技术的研究进展

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CRISPR 传感检测技术具有便宜、简单、便携、高灵敏和高特异等优点,被称为"下一代分子诊断技术".由于 CRISPR-Cas 系统具有特异的识别、顺式切割和非特异性的反式切割能力,已经实现了对DNA和RNA等核酸靶标以及蛋白质、外泌体、细胞和小分子等非核酸靶标的检测.为了解不同CRISPR传感检测技术的优势和发展历程,促进该技术的发展和应用,本文根据不同Cas蛋白的活性特征,对目前的CRISPR传感检测技术进行了分类总结,并在此基础上根据检测的靶标类型,依次总结了各种CRISPR传感检测技术的应用情况,以期为开发新型CRISPR传感检测技术提供参考.
Advances in CRISPR sensing and detection technology
The CRISPR sensing and detection technology has the advantages of cheap,simple,portable,high sensitivity,and high specificity,therefore is regarded as the"next-generation molecular diagnostic technology".Due to the specific recognition,cis-cleavage and nonspecific trans-cleavage capabilities,CRISPR-Cas systems have been implemented for the detection of nucleic acid targets(DNA and RNA)as well as non-nucleic acid targets(e.g.,proteins,exosomes,cells,and small molecules).This review summarizes the current CRISPR sensing and detection technologies in terms of the activity characteristics of different Cas proteins,with the aim to understand the advantages and development history of different CRISPR sensing and detection technologies,as well as promote its development and application.Moreover,this review summarizes the applications of various CRISPR sensing and detection technologies according to the types of detection targets,hoping to facilitate the development of novel CRISPR sensing detection technology.

clustered regulatory interspaced short palindromic repeats(CRISPR)biosensorsinstant detectionmolecular diagnostics

王佳慧、赵佩佩、秦梦飞、赵燕秋、刘成伟、夏雪奎

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齐鲁工业大学(山东省科学院)生物研究所,山东 济南 250103

黑龙江大学现代农业与生态环境学院,黑龙江 哈尔滨 150080

东北林业大学生命科学学院,黑龙江 哈尔滨 150040

规律间隔成簇短回文重复序列(CRISPR) 生物传感器 即时检测 分子诊断

山东省重点研发计划山东省自然科学基金创新发展联合基金泰山学者青年专家岗位齐鲁工业大学(山东省科学院)重大创新专项齐鲁工业大学(山东省科学院)科教产融合试点工程基础研究类项目齐鲁工业大学(山东省科学院)科教产融合试点工程基础研究类项目山东省自然科学基金青年基金

2022SFGC0105ZR2021LSW022tsqn2021031002022JBZ01-062022PX0512022PX027ZR2022QC186

2024

10.13345/j.cjb.230482

生物工程学报
中国科学院微生物研究所 中国微生物学会

生物工程学报

CSTPCD北大核心
影响因子：0.641
ISSN：1000-3061
年,卷(期)：2024.40(2)