摘要
本研究旨在探究草珊瑚叶和根中萜类化合物的组织特异性分布差异,解析其药效品质差异形成的分子机制.采用液相色谱-质谱联用技术(liquid chromatography-mass spectrometry,LC-MS)和Illumina HiSeqTM高通量测序技术获得草珊瑚[Sarcandra glabra(thunb)naka]叶和根的代谢组学和转录组学数据.代谢组学结果表明参与叶和根中萜类合成的差异代谢物有 50 个,包括法尼西基半胱氨酸、甘油醛-3-磷酸、甲羟戊酸-5-磷酸等.转录学结果表明差异代谢酶基因有 57 条,包括ACTC、HMGCR、MVK、DXS与KS等,并预测了MYB、C2H2、AP2/ERF-ERF等 7 个转录因子参与调控草珊瑚不同组织部位中萜类的合成和积累差异.实时荧光定量 PCR(quantitative real-time polymerase chain reaction,qRT-PCR)结果显示,随机选取的 8 个参与萜类合成的酶基因在草珊瑚不同组织部位中的表达趋势与转录组学测序结果一致.本研究有助于阐明草珊瑚叶和根临床疗效差异形成的分子机制,同时为草珊瑚的资源开发利用奠定基础.
Abstract
The leaves and roots of Sarcandra glabra(thunb)nakai have different therapeutic effects in some clinical applications.In order to explore the tissue specific distribution differences of terpenoids in the leaves and roots of S.glabra,and to analyze the molecular mechanism of the formation of their pharmacodynamic quality differences.In this study,liquid chromatography-mass spectrometry(LC-MS)and Illumina HiSeqTM high-throughput sequencing techniques were respectively used to obtain the metabolome and transcriptome data of the leaves and roots of S.glabra.The metabolomics analysis showed that there were 50 differential terpenoids metabolites between the leaves and roots,including farnesylcysteine,D-glyceraldehyde 3-phosphate,and(R)-5-phosphomevalonate.The transcriptomics analysis indicated that there were 57 differentially expressed metabolic enzyme coding genes,including ACTC,HMGCR,MVK,DXS,and KS.Moreover,there were seven transcription factors,including MYB,C2H2,AP2/ERF-ERF,which were predicted to participate in regulating the differences in terpenoid synthesis and accumulation between the leaves and roots of S.glabra.qRT-PCR results demonstrated that the expression changes of eight randomly selected enzyme genes involved in terpene synthesis between the leaves and roots of S.glabra,which were consistent with the transcriptome sequencing results.This study will help to elucidate the molecular mechanisms underlying the clinical efficacy differences between the leaves and roots of S.glabra,and facilitate the extraction,utilization,and resource development of S.glabra.
维普
万方数据