Screening and identification of host proteins interacting with the non-structural protein 15(Nsp15)of porcine epidemic diarrhea virus
To screen and identify the key host proteins interacting with the non-structural protein 15(Nsp15)of porcine epidemic diarrhea virus(PEDV).The IP/pull-down assay and mass spectrometry were employed to screen and identify the host proteins interacting with Nsp15.The interaction between the host protein and Nsp15 was studied by co-immunoprecipitation and laser scanning confocal microscopy.Finally,Western blotting and RT-qPCR were employed to examine the interaction between SLC25a3 and PEDV.The recombinant eukaryotic expression vector pcDNA3.1(+)-Flag-Nsp15 was successfully constructed,and the host protein SLC25a3 interacting with PEDV Nsp15 was screened out.An interaction existed between SLC25a3 and Nsp15,and SLC25a3 significantly inhibited PEDV replication in a dose-dependent manner.SLC25a3 inhibits PEDV replication.The results of this study provide a basis for deciphering the role and mechanism of SLC25a3 in the host immune response to PEDV infection.
万方数据
维普
