Genetic diversity and structure of 15 full-sib families of Litopenaeus vannamei based on SSR markers
To clarify the genetic diversity and structure of the nucleus population of F1-generation Litopenaeus vannamei,this study utilized 15 pairs of highly polymorphic microsatellite primers to analyze the simple sequence repeat(SSR)markers and genetic diversity in 15 full-sib families of L.vannamei.A total of 112 alleles(Na)and 60.453 effective alleles(Ne)were identified among the selected 15 SSR loci,with the average polymorphic information content(PIC)of 0.648.The average Ne,observed heterozygosity(Ho),and expected heterozygosity(He)in the 15 F1 families varied from 1.925 to 2.626,0.425 to 0.783,and 0.403 to 0.572,respectively.The 15 full-sib families were primarily clustered into three categories in the phylogenetic analysis,with the genetic distance between families ranging from 0.252 to 0.574.Additionally,the genetic differentiation coefficient(Fst)among the families varied from 0.112 to 0.278,indicating substantial genetic differentiation.Overall,this study suggested that the genetic diversity of the 15 full-sib families was moderate,providing valuable genetic insights for the subsequent breeding initiatives aimed at enhancing the tolerance of L.vannamei to high levels of soybean meal.
万方数据
维普
