Blocking IFN-β Improves the T Lymphocyte Proliferation Inhibitions Induced by Lipopolysaccharide
Objective:To explore the effect of inflammatory cytokines on the immunosuppression of T lymphocytes in lipopolysaccharide bacterial sepsis.Methods:Gram-negative bacterial component lipopolysaccharide is used to induce dendritic cells to replicate the immuno-suppressive model of bacterial sepsis.Flow cytometry is used to detect the expression of inflammatory cytokines Interleukin-1α(IL-1α),IL-6,tumor necrosis factor-α(TNF-α),IL-10,transforming growth factor-β(TGF-β),interferon-α(IFN-α),IFN-β of dendritic cells in the control group and model group.Then,the BrdU cell proliferation detection kit is used to detect the reversal of immune proliferation inhibition of T lymphocytes in mixed lymphocyte coculture experiments.Results:Compared with the control group,the positive percentages of the inflammatory cytokines IL-1α,IL-6,TNF-α,TGF-β and IFN-β of the dendritic cells in the model group are all significantly increased.But there is no statistical difference of IL-10,IFN-α.Compared with the model group,the T cell proliferation response of the αIFN-β+model group are significantly increased.Conclusion:The up-regulation of inflammatory cytokines IL-1α,IL-6 and TNF-α in dendritic cells may be involved in mediating the pro-inflammatory response process in bacterial sepsis.IFN-β upregulation may be involved in the lymphocyte apoptosis of immune suppression process in sepsis.Importantly IFN-β blocking can significantly reverse the inhibition of T lymphocyte proliferation in lipopolysaccharide-induced immuno-paralysis.
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