莪术、郁金及近缘品种UPLC特征图谱研究
Study on UPLC Specific Chromatogram of Curcuma zedoaria,Curcuma aromatica and Related Varieties
张玉玮 1张金龙 1李广华 1张明娟1
作者信息
- 1. 聊城市检验检测中心,山东聊城 252000
- 折叠
摘要
目的:建立超高效液相色谱特征图谱方法,对莪术、郁金及近缘品种进行鉴别.方法:采用超高效液相色谱法,以乙腈-0.1%磷酸溶液为流动相进行梯度洗脱,Luna Omega C18 色谱柱(150 mm×2.1 mm,1.6 μm),流速 0.30 mL/min,检测波长 230 nm,柱温 35℃.结果:建立了超高效液相色谱特征图谱方法,共识别出 6 个特征峰,可根据特征峰的多少及相对峰面积的大小对各品种进行鉴别.结论:建立的UPLC特征图谱方法特征性强,稳定性及重复性好,为莪术、郁金及近缘品种的鉴别及质量控制提供了科学便捷的方法和依据.
Abstract
Objective:To establish the UPLC specific chromatogram and a method of UPLC specific chromatogram is developed to identify Curcuma zedoaria,Curcuma aromatica and related varieties.Methods:Using ultra-high performance liquid chromatography with acetonitrile 0.1%phosphoric acid solution as mobile phase for gradient elution,Luna Omega C18 column(150 mm×2.1 mm,1.6 μm)is used,the flow rate of 0.30 mL/min,detection wavelength of 230 nm,column temperature of 35℃.Results:A total of 6 characteristic peaks are detected by the established UPLC specific chromatogram,and each variety is identified according to the number of characteristic peaks and the relative peak area of characteristic peaks.Conclusion:The established UPLC feature map method has strong characteristics,good stability and repeatability,providing a scientific and convenient method and basis for the identification and quality control of Curcuma zedoaria,Curcuma aromatica and related varieties.
关键词
莪术/郁金/近缘品种/超高效液相色谱/特征图谱Key words
Curcuma zedoaria/Curcuma aromatica/related varieties/UPLC/characteristic chromatogram引用本文复制引用
出版年
2024
NETL
NSTL
万方数据