Study on UPLC Specific Chromatogram of Curcuma zedoaria,Curcuma aromatica and Related Varieties
Objective:To establish the UPLC specific chromatogram and a method of UPLC specific chromatogram is developed to identify Curcuma zedoaria,Curcuma aromatica and related varieties.Methods:Using ultra-high performance liquid chromatography with acetonitrile 0.1%phosphoric acid solution as mobile phase for gradient elution,Luna Omega C18 column(150 mm×2.1 mm,1.6 μm)is used,the flow rate of 0.30 mL/min,detection wavelength of 230 nm,column temperature of 35℃.Results:A total of 6 characteristic peaks are detected by the established UPLC specific chromatogram,and each variety is identified according to the number of characteristic peaks and the relative peak area of characteristic peaks.Conclusion:The established UPLC feature map method has strong characteristics,good stability and repeatability,providing a scientific and convenient method and basis for the identification and quality control of Curcuma zedoaria,Curcuma aromatica and related varieties.
NETL
NSTL
万方数据
