Determination of Levamisole Hydrochloride in Oral Solution of Health Care Products by HPLC
Objective:To establish an HPLC method for detecting levamisole hydrochloride in oral health products.Methods:Using a C18-WR chromatography column(4.6 mm×250 mm,5 μm),the mobile phase is acetonitrile-0.05 mol/L potassium dihydrogen phosphate solution(pH=7 adjusted with triethylamine)(25∶75,volume ratio),with a flow rate of 1.0 mL/min,detection wavelength of 210 nm,column temperature of 30℃,and injection volume of 10 μL.Results:The linear relationship between levamisole hydrochloride and peak area is good in the range of 8.634 μg/mL to 86.340 μg/mL(R=0.9997);the average recovery rates corresponding to low,medium,and high spiked quantities are 99.7%,99.2%,and 100.3%,respectively,with RSD of 1.40%,0.64%,and 0.78%,and detection limit of 0.4317 μg/mL.Randomly tested 16 batches of commercially available samples,but no levamisole hydrochloride is detected.Conclusion:This method is simple,fast,and accurate,and can be used for the detection of illegal addition of levamisole hydrochloride in oral health products.
oral solution of health care productslevamisole hydrochlorideHPLC
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