Seed germination is the initial stage of plant growth and an important period of susceptibility to abiotic stress,including drought.Proteomics is one of the major tools for analyzing plant responses to stress,and two-dimensional electrophoresis separation is an important means and step in proteomics.In the study,the radicle proteins of"Chunbulao"(Raphanus sativus L.)during germination period under drought stress simulating by Polyethylene Glycol-600(PEG-6000)are separated by two-dimensional electrophoresis.By directly lysising and loading radish non-cotyledons,a two-dimensional electrophoresis protein map with good resolution and repeatability is obtained;differential analysis is conducted on the expression profile of non-cotyledon proteins in radish during seed germination under drought stress,a total of 265 radish protein points are detected and matched,with 28 protein points responded to drought stress,of which 13 are significantly upregulated and 15 are significantly downregulated.The research results indicate that a few of radish seed germination proteins respond to drought stress and laid a solid foundation for subsequent protein mass spectrometry analysis and identification of responsive proteins.In addition,the method of direct lysis of samples used in this study also provides reference for two-dimensional electrophoresis analysis of other plant proteins.
NETL
NSTL
万方数据
