Objective:To study the killing effect on tumor of lymphocytes sensitized by tumor antigen plused with Ginkgo biloba exocarp extracts(GBEE)intervention.Methods:Using normal mice's spleen prepare spleen lymphocytes suspension(Lc);tumor antigen is prepared from supernatant by repetitive freeze thaw method(Ag);tumor antigen-sensitized lymphocyte solution(AgLc)prepared with the volume ratio of spleen lymphocytes to tumor antigens is 2 to 1,and then mixed with 480 μg/mL concentrations of GBEE.After incubation,it's added to a 6-well plate seeded with Heps cells and cultured,which is the GBEE+AgLc+Heps group,while Heps group,Lc+Heps group,AgLc+Heps group,GBEE+Heps group,GBEE+Lc+Heps group are setted.Killing effects on Heps cells of lymphocytes in vitro are measured by MTT assays.Results:The killing effect of lymphocytes sensitized by liver cancer antigen is stronger,killing rate is 10.63%,compared with Lc+Heps group,P＜0.05.GBEE + AgLc + Heps group is the most obvious effect of killing tumor cells,killing rate is 50.59%,compared with all the groups,P＜0.05.Conclusion:GBEE can promote tumor antigen sensitized lymphocytes killing effect on tumor cells.
Ginkgo biloba exocarp extractsspleen lymphocytesliver cancer cellsantigen
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