侧柏叶有效成分提取及酪氨酸酶活性影响评价
Extraction of Effective Components from Platycladus orientalis Leaves and Evaluation of the Effect of Tyrosinase Activity
简嘉 1刘雪洁 1郝祥羽 1杨昊 1佟显意 1吴志锦 1续乘轩1
作者信息
摘要
目的:研究侧柏叶有效成分总黄酮的最佳提取工艺,并评价有效提取成分对酪氨酸酶活性的影响.方法:采用单因素试验、正交试验考察了提取过程中各因素对侧柏叶总黄酮含量的影响;采用蘑菇酪氨酸酶多巴速率氧化法考察提取成分对酪氨酸酶活性的影响.结果:本实验确定最优的提取工艺为 90%乙醇作为提取液,按照 1∶15 的料液比(g∶mL),以 70℃的超声温度进行提取,超声时间为 60 min,此条件下总黄铜提取量为 1.394 mg/g.0.5 g/mL乙酸乙酯提取物的激活率最小,为-211.68%±2.42%;0.5 g/mL石油醚提取物的激活率最大,为 20.30%±1.32%.结论:本研究确定了侧柏叶有效成分提取的最佳工艺,经过对有效成分活性测试可知,乙醇粗提取物和乙酸乙酯提取物对酪氨酸酶的活性有抑制作用且随着浓度升高而升高,石油醚提取物低浓度有抑制作用,高浓度有激活作用.
Abstract
Objective:To study the optimal extraction process of total flavonoids,an effective component of Platycladus orientalis leaves,and evaluate the effect of effective component on tyrosinase activity.Methods:Single factor experiments and orthogonal experiments are used to investigate the effects of various factors during the extraction process on the total flavonoid content of Platycladus orientalis leaves;the mushroom tyrosinase dopa rate oxidation method is used to investigate the activity of effective component on tyrosinase.Results:The optimal extraction process is as follows:90%ethanol in the extraction solution,a material to liquid ratio of 1 to 15(g ∶ mL),ultrasound temperature of 70℃,and ultrasound time of 60 min.The total brass extraction under these conditions is 1.394 mg/g.The activation rate of 0.5 g/mL ethyl acetate extract is the lowest,which is-211.68%±2.42%,and the activation rate of 0.5 g/mL petroleum ether extract is the highest,which is 20.30%±1.32%.Conclusions:This experiment has determined the optimal process for extracting the active ingredients from the leaves of Platycladus orientalis.After testing the activity of the active ingredients,it is found that the crude ethanol extract and ethyl acetate extract have inhibitory effects on the activity of tyrosinase and increase with increasing concentration.The petroleum ether extract has inhibitory effects at low concentrations,and activation effects at high concentrations.
关键词
侧柏叶/工艺优化/提取物/酪氨酸酶活性Key words
Platycladus orientalis leaves/process optimization/extract/tyrosinase activity引用本文复制引用
基金项目
国家级大学生创新创业训练计划项目(202210859004)
出版年
2024
NETL
NSTL
万方数据