Objective:To study the optimal extraction process of total flavonoids,an effective component of Platycladus orientalis leaves,and evaluate the effect of effective component on tyrosinase activity.Methods:Single factor experiments and orthogonal experiments are used to investigate the effects of various factors during the extraction process on the total flavonoid content of Platycladus orientalis leaves;the mushroom tyrosinase dopa rate oxidation method is used to investigate the activity of effective component on tyrosinase.Results:The optimal extraction process is as follows:90%ethanol in the extraction solution,a material to liquid ratio of 1 to 15(g ∶ mL),ultrasound temperature of 70℃,and ultrasound time of 60 min.The total brass extraction under these conditions is 1.394 mg/g.The activation rate of 0.5 g/mL ethyl acetate extract is the lowest,which is-211.68%±2.42%,and the activation rate of 0.5 g/mL petroleum ether extract is the highest,which is 20.30%±1.32%.Conclusions:This experiment has determined the optimal process for extracting the active ingredients from the leaves of Platycladus orientalis.After testing the activity of the active ingredients,it is found that the crude ethanol extract and ethyl acetate extract have inhibitory effects on the activity of tyrosinase and increase with increasing concentration.The petroleum ether extract has inhibitory effects at low concentrations,and activation effects at high concentrations.
Platycladus orientalis leavesprocess optimizationextracttyrosinase activity
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