Objective:To study the optimal process for the extraction of α-amylase inhibitors from white kidney beans.Methods:The effects of fineness,extraction time,extraction temperature,ultrasonic power and solid-liquid ratio on the yield of α-amylase inhibitors in white kidney beans are studied by ultrasound-assisted extraction.Results:Through single factor and orthogonal experiments,the optimal process conditions are determined to be as follows:the ratio of solid to liquid of 1 to 10(g to mL),extraction temperature of 60℃,extraction time of 100 min,and ultrasonic power of 200 W.The repeated test is performed according to the optimized conditions,and the yield of α-amylase inhibitor in white kidney beans is 4.51%.
white kidney beansα-amylase inhibitorultrasonic-assisted extractionprocess optimization
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