Objective:To establish a method for the determination of gallic acid in Wine Paeoniae Radix Alba.Methods:HPLC is employed to determine the content and quality evaluation of gallic acid in Wine Paeoniae Radix Alba.Results:The method for determination of the gallic acid is stable,and the linear relationship between peak area and mass concentration of main components is good(R>0.9999),whose average recoveries is 97.6%,the RSD is 0.7%.Conclusion:A method for determination of gallic acid in Wine Paeoniae Radix Alba is established,which can provide experimental basis for quality control of Wine Paeoniae Radix Alba.