This article presents a comprehensive overview of recent advancements in in vivo imaging of cortical neurons in large animals.The study of large animal cortical neurons is crucial for understanding brain function,thus making the in vivo imaging of these neurons of great significance.The focus of this article is on emerging techniques such as magnetic resonance imaging(MRI),electrophysiological methods,and optical imaging,which have surfaced in recent years.These innovative techniques have improved the resolution and depth of neuronal imaging,allowing for real-time tracking of neuronal activity.Additionally,the applications of these techniques in studying large animal cortical neurons are discussed,along with an analysis of future trends in their development,which aims to provide researchers in this field with a comprehensive overview of in vivo imaging of large animal cortical neurons,serving as a valuable reference and guide.
large animalscortical neuronsin vivo imagingmultiphoton imaging