Acyl-coenzyme A,as an important form of acyl activation,plays a crucial role in metabolism,and obtaining acyl-coenzyme A is the basis for studying related metabolic processes.Currently,the synthesis methods of acyl-coenzyme A mainly include chemical synthesis and enzymatic synthesis,and the applicable synthesis methods vary for different structures of acyl-coenzyme A.In this paper,chemical synthesis,namely anhydride synthesis,imidazole activation,and PyBOP one-step synthesis,and enzymatic synthesis,namely CoA ligase and acyl transferase complete in vitro catalysis are used to synthesize phenyllactyl coenzyme A,isovaleryl coenzyme A,benzoyl coenzyme A,coumaroyl coenzyme A,cinnamoyl coenzyme A respectively,and the synthesis results are evaluated.The results show that enzymatic method has the advantages of high conversion rate and high specificity,and chemical synthesis has the characteristics of efficient and rapid synthesis.This paper provides a reference for the rational selection of acyl-coenzyme synthesis methods in the future.
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