Study on Bacteriostatic Mechanism of Clove and Cinnamon Essential Oils Against Three Common Pathogenic Bacteria
By used kit method,nucleic acid gel electrophoresis,fluorescence staining observation and other research methods,the antibacterial mechanism of the clove and cinnamon essential oils is revealed from the changes of extracellular sugar and protein content,the activity of key enzymes in the cellular respiratory chain and DNA double strand integrity.The results show that clove essential oil and cinnamon essential oil could cause macromolecular leakage of sugar and protein,DNA damage and decreased activity of intracellular succinate dehydrogenase in Escherichia coli,Staphylococcus aureus and Candida albicans;clove essential oil significantly damaged the integrity of cell wall,cell membrane and DNA structure,while cinnamon essential oil is more active in inhibiting the activities of metabolic key enzymes and interfering with respiration.The results of this study fully confirmed the potential of the clove essential oil and cinnamon essential oils as components of a new natural preservative system.
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