玉竹多糖对人脐带间质干细胞培养的影响研究
Study on the Influence of Polysaccharide of Polygonatum odoratum on the Growth of Human Umbilical Cord Mesenchymal Stem Cell
宋泓纬 1高业胜 2江晔2
作者信息
- 1. 湖南农业大学 生物科学技术学院,湖南长沙 410000
- 2. 华颜医药科技有限公司,上海 200020
- 折叠
摘要
目的:探究培养液中添加玉竹多糖对人脐带间质干细胞(hUC-MSCs)培养的影响.方法:比较在培养液中添加玉竹多糖和传统培养方法得到的人脐带间质干细胞,在形态、稳定性、产量、细胞分化等指标上的区别.结果:测得传统酶消化法P0 代细胞每瓶(T75)收获细胞总量为 4.53×106 cells、活率 95.5%,而添加玉竹多糖的酶消化法收获细胞总量为 5.35×107 cells、活率 97.9%;定向诱导分化两种方法得到的hUC-MSCs,无明显差异.结论:与传统酶消化法相比,添加玉竹多糖的酶消化法可以提高细胞产量、缩短生产时间,利于细胞的大规模化生产,且细胞分化无明显差异,能满足生产和临床需求.
Abstract
Objective:To investigate the effect of polysaccharide of Polygonatum odoratum in culture medium on human umbilical cord mesenchymal stem cells(hUC-MSCs)culture.Methods:Compare the morphological,stability and yield of human umbilical cord mesenchymal stem cells obtained by adding polysaccharide of Polygonatum odoratum in culture medium and traditional culture method.Results:The total number of cells harvested per vial(T75)by the traditional enzyme digestion method is 4.53×106 cells and the viability rate is 95.5%,while the total number of cells harvested by the enzyme digestion method with polysaccharide of Polygonatum odoratum is 5.35×107 cells and the viability rate is 97.9%;there is no significant difference between hUC-MSCs obtained by the two methods of directional induction differentiation.Conclusion:Compared with the traditional enzyme digestion method,the enzyme digestion method with polysaccharide of Polygonatum odoratum can increase the cell yield and shorten the production time,which is conducive to the large-scale production of cells,and there is no significant difference in cell differentiation,which could meet the production and clinical needs.
关键词
人脐带间充质干细胞/细胞培养/酶消化法/玉竹多糖Key words
human umbilical cord mesenchymal stem cells/cell culture/enzyme digestion method/polysaccharide of Polygonatum odoratum引用本文复制引用
出版年
2024
NETL
NSTL
万方数据