Objective:To investigate the effect of polysaccharide of Polygonatum odoratum in culture medium on human umbilical cord mesenchymal stem cells(hUC-MSCs)culture.Methods:Compare the morphological,stability and yield of human umbilical cord mesenchymal stem cells obtained by adding polysaccharide of Polygonatum odoratum in culture medium and traditional culture method.Results:The total number of cells harvested per vial(T75)by the traditional enzyme digestion method is 4.53×106 cells and the viability rate is 95.5%,while the total number of cells harvested by the enzyme digestion method with polysaccharide of Polygonatum odoratum is 5.35×107 cells and the viability rate is 97.9%;there is no significant difference between hUC-MSCs obtained by the two methods of directional induction differentiation.Conclusion:Compared with the traditional enzyme digestion method,the enzyme digestion method with polysaccharide of Polygonatum odoratum can increase the cell yield and shorten the production time,which is conducive to the large-scale production of cells,and there is no significant difference in cell differentiation,which could meet the production and clinical needs.
human umbilical cord mesenchymal stem cellscell cultureenzyme digestion methodpolysaccharide of Polygonatum odoratum
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