Objective:Using URAT1(uric acid transporter 1)as the target,molecular docking is used to screen the effective ingredients in three prescriptions that inhibit URAT1.Methods:The URAT1 is modelled using AlphaFold2.1.The components of Smilax glabra,Clematis chinensis Osbeck and Dioscorea tokoro Makino are docked with URAT1 using ledock software,and compared with the drug benzbromarone to select the components with better activity.Results:The docking results show that multiple components in the three traditional Chinese medicines can bind well with the URAT1 active pocket,forming multiple hydrogen bonds,and some even form π-π bonds.Among them,22 components such as simiglaside B and enbinin have better docking scores than benzbromarone.Conclusion:The three prescriptions contains 22 components,such as tucoside B and enbinin,which molecular docking results are superior to the benzbromarone.Therefore,this prescription can inhibit URAT1 and reduce uric acid concentration.Among the three traditional Chinese medicines,Smilax glabra rhizome contains the most active ingredients.
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