Objective:To study the chemical composition of ethyl acetate layer in Salvia plebeia.Methods:Silica gel column chromatography,medium-pressure rapid chromatography and Sephadex-LH gel chromatography are used for separation and purification,and the structure of each monomer compound is identified by spectroscopy,physicochemical properties and literature control.Results:Two monomeric compounds are isolated from the ethyl acetate layer of Salvia plebeia,which are identified as zealand flavonoids and homopsyllin,respectively.Conclusion:For the first time,medium-pressure chromatography is used to quickly obtain zealand flavonoids and homopsyllin from the ethyl acetate extraction layer of Salvia plebeia,which is time-saving,simple and efficient,and worthy of popularization and application.
Salvia plebeiaeupafolinhispidulinmedium pressure column chromatography
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