Objective:Determination of tanshinone ⅡA in Salvia castanea Diels f.tomentosa Stib.in four resource-rich areas of Linzhi city by high performance liquid chromatography.Methods:The chromatographic conditions are as follows:Supersil ODS2-C18 column(4.6 mm×250 mm,5 μm),acetonitrile-0.02%phosphoric acid solution as gradient eluent mobile phase,flow rate at 1 mL/min,20℃constant column temperature,detection wavelength of 270 nm,injection volume of 10 μL.Tanshinone ⅡA in Salvia castanea Diels f.tomentosa Stib.is extracted by ultrasonic extraction and alcohol extraction and water sedimentation method,respectively,and the content differences between the two methods and different origins are compared.Results:The experimental results show that the two methods of ultrasonic e xtraction and alcoholic precipitation could extract tanshinone ⅡA,and the content of alcohol extraction and water precipitation is higher,which is more suitable for the industrialized production of tanshinone ⅡA.The content of the four advantageous production areas is tested,in which,the sample content of Milin city is relatively stable,and the sample content in Lang county was even higher.Conclusion:This method is simple,accurate,efficient and stable,which provides a reference for the resource development and cultivation of Salvia castanea Diels f.tomentosa Stib.and a scientific basis for the full utilization of Salvia castanea Diels f.tomentosa Stib.resources.
NETL
NSTL
万方数据
