Biological laboratory is an important base for biological experiments and personnel training in colleges and universities.However,because biological laboratory involves many types of experiments,complex experimental materials,and some equipment and chemical reagents have high risk,the safety management of biological laboratory is facing great challenges.Once the safety accident occurs,it will not only affect the normal teaching of colleges and universities,but also endanger the health and life safety of teachers and students.Based on this,this paper will focus on the hazard sources of biological laboratories in colleges and universities,and improve the laboratory safety index from strengthening safety education and training,increase investment in safety facilities,optimize the construction of laboratory ventilation systems,establish laboratory dangerous goods files and strengthening laboratory waste treatment and other aspects.