Selection of High-Yield Abscisic Acid Strains Through Atmospheric Room Temperature Plasma Mutagenesis
Objective:To utilize atmospheric room temperature plasma(ARTP)technology to induce mutations in Botrytis cinerea,a potential host for abscisic acid production,aiming to obtain genetically stable mutant strains with high abscisic acid titer.Methods:A mixed enzyme solution is used to remove the cell wall of B.cinerea to obtain protoplasts.The protoplasts are then subjected to ARTP mutagenesis treatment under conditions of 120 W output power,10 L/min working gas flow,2 mm irradiation distance,and 120 s irradiation time.Finally,the abscisic acid yield of the mutant strains is measured,and fermentation optimization is performed on the best mutant strain.Results:Compared to the wild-type strain,the mutant strains show varying degrees of improvement in abscisic acid production.Notably,mutant strains A4 and A7 exhibit significant increases in abscisic acid production,with enhancements of 26.3%and 30.5%,respectively.Further single-factor experiments determined that the optimal fermentation carbon and nitrogen sources for mutant strain A7 are glucose and soybean meal,with an optimal inoculation amount of 8%.Conclusion:The mutant strains with high abscisic acid production can be effectively obtained by using ARTP mutagenesis technology,which provides favorable support for the industrial production of abscisic acid.
abscisic acidBotrytis cinereaatmospheric room temperature plasmaabscisic acid production level
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