ARTP诱变灰葡萄孢霉筛选高产脱落酸菌株
Selection of High-Yield Abscisic Acid Strains Through Atmospheric Room Temperature Plasma Mutagenesis
周金龙 1廖海兵 1毛玉华 1卢山 1雷文 1聂志奎1
作者信息
- 1. 江西新瑞丰生化股份有限公司,江西新干 331300
- 折叠
摘要
目的:利用常压室温等离子体(Atmospheric Room Temperature Plasma,ARTP)技术对生产脱落菌的潜力宿主灰葡萄孢霉进行诱变,以获得遗传稳定性好的高产脱落酸的突变菌种.方法:利用酶解法去除灰葡萄孢霉细胞壁,从而获得灰葡萄孢霉原生质体.接着将灰葡萄孢霉原生质体在输出功率 120 W,工作气流 10 L/min,照射距离 2 mm,照射时间 120 s的条件下进行ARTP诱变处理.检测突变菌株的脱落酸产量,并对最佳突变菌株进行发酵优化.结果:与野生型相比,突变菌株的脱落酸生产能力有了不同程度的提高,其中突变菌株A4 和A7 的脱落酸生产能力显著提高,分别提高了 26.3%和 30.5%.进一步通过单因素实验,确定突变菌株A7 的最佳发酵碳源和氮源成分为葡萄糖和黄豆粉,最佳接种量为 8%.结论:采用ARTP诱变技术能够有效获得高产脱落酸的突变菌株,为脱落酸的工业化生产提供了有利支撑.
Abstract
Objective:To utilize atmospheric room temperature plasma(ARTP)technology to induce mutations in Botrytis cinerea,a potential host for abscisic acid production,aiming to obtain genetically stable mutant strains with high abscisic acid titer.Methods:A mixed enzyme solution is used to remove the cell wall of B.cinerea to obtain protoplasts.The protoplasts are then subjected to ARTP mutagenesis treatment under conditions of 120 W output power,10 L/min working gas flow,2 mm irradiation distance,and 120 s irradiation time.Finally,the abscisic acid yield of the mutant strains is measured,and fermentation optimization is performed on the best mutant strain.Results:Compared to the wild-type strain,the mutant strains show varying degrees of improvement in abscisic acid production.Notably,mutant strains A4 and A7 exhibit significant increases in abscisic acid production,with enhancements of 26.3%and 30.5%,respectively.Further single-factor experiments determined that the optimal fermentation carbon and nitrogen sources for mutant strain A7 are glucose and soybean meal,with an optimal inoculation amount of 8%.Conclusion:The mutant strains with high abscisic acid production can be effectively obtained by using ARTP mutagenesis technology,which provides favorable support for the industrial production of abscisic acid.
关键词
脱落酸/灰葡萄孢霉/常压室温等离子体/脱落酸生产水平Key words
abscisic acid/Botrytis cinerea/atmospheric room temperature plasma/abscisic acid production level引用本文复制引用
基金项目
江西省赣鄱俊才支持计划主要学科学术和技术带头人培养项目(20232BCJ23072)
江西省地质局科技研究项目(2022JXDZKJKY12)
出版年
2024
NETL
NSTL
万方数据