摘要
为了研究黄花倒水莲花的化学成分及其降胆固醇活性,本文采用硅胶、MCI、Sephadex LH-20 和高效液相色谱等技术对黄花倒水莲花的 95%乙醇提取物低极性部位进行分离纯化,共得到 11 个化合物,根据波谱数据及文献比对,分别鉴定为 3,4-二甲氧基肉桂酸(1)、1,3,6-三羟基-2,7-二甲氧基口山酮(2)、7-羟基-1-甲氧基-2,3-亚甲二氧基口山酮(3)、1,7-二羟基-2,3-亚甲二氧基口山酮(4)、1-甲氧基-2,3-亚甲二氧基口山酮(5)、1,3-二羟基-2-甲氧基口山酮(6)、(2S,3S,4R,10E)-2-[(2R)-2-羟基-二十四烷酰氨基]-10-十八烷-1,3,4-三醇(7)、楤木脑苷脂(8)、波甾醇-3-O-β-D-吡喃葡萄糖苷(9)、棕榈酸(10)、硬脂酸(11),其中化合物 7 和 9 为首次从该植物中分离得到.采用试剂盒法测定所获得化合物对HepG2细胞胆固醇含量的影响,活性筛选结果显示化合物8具有良好的降胆固醇活性,半抑制浓度(IC50)为40.3 µmol/L,可作为降胆固醇药物研发的候选分子.
Abstract
This work is to investigate the chemical constituents and their cholesterol-lowering activity of flowers of Polygala fallax.Eleven compounds are isolated and purified through silica gel,MCI,Sephadex LH-20 and high-performance liquid chromatography(HPLC)from the low polarity components of 95%ethanol extract of flowers of Polygala fallax.Based on the spectral data and literature comparison,their chemical structures are identified as 3,4-dimethoxy cinnamic acid(1),1,3,6-trihydroxy-2,7-dimethoxyxanthone(2),7-hydroxy-1-methoxy-2,3-methylenedioxyxanthone(3),1,7-dihydroxy-2,3-methylenedioxyxanthone(4),1-methoxy-2,3-methylenedioxyxanthone(5),1,3-dihydroxy-2-methoxyxanthone(6),(2S,3S,4R,10E)-2-[(2R)-2-hydroxyl-tetracosanoly-amino]-10-octadecene-1,3,4-triol(7),aralia cerebroside(8),3-O-β-D-glucopyranosyl spinasterol(9),palmitic acid(10),stearic acid(11),respectively.Compounds 7 and 9 are isolated from this plant for the first time.The effect of the obtained compounds on the cholesterol content are determined using the kit method in HepG2 cells,and the activity screening results show that compound 8 has good cholesterol-lowering activity with an half maximal inhibitory concentration(IC50)of 40.3 μmol/L,which can be used as a candidate molecule for the development of cholesterol-lowering drugs.
基金项目
国家自然科学基金(82360685)
广西科技重大专项(桂科AA22096020)
广西林业科技推广示范项目(桂林科研[2022]第3号)
中国科学院西部青年学者(人字[2023]16号)
桂林市创新平台和人才计划(20210102-3)
NETL
NSTL
万方数据